Transfection Reagents
Lonza Walkersville Mouse Neural Stem Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary mouse neural stem cells (NSCs).The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Neural Stem Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Cell Line Nucleofector Kit R
The Cell Line Nucleofector Kit R is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:B16-F0BJCOS-7HeLaHT-29Hut-78IMR-90KG-1L6LNCaPMDBKNIH/3T3NK-92PANC-1Sf9WI-38 The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution R0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Cell Line Nucleofector Kit L
The Cell Line Nucleofector Kit L is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device.This kit has been determined to be optimal for transfecting following cell types:3T3-L1 adA-10BHK-21BxPC-3DU145EL4FDC-P1GH3 H9c2 (2-1)HeLa S3IMR-32KG-1aL-428 (DSMZ)MDCKMDCK II (ECACC)MOLT-4MV-4-11NTERA-2 cl.D1WEHI-231The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format
Genesee Scientific Corporation TRANSFECTION REAGENT 0.1ML
0.1ml Reagent with 10ml Buffer0.1ml/UnitBrand: Polyplus-transfectionBest-in-Class DNA Transfection ReagentBased on Novel Cationic NanotechnologyReach Maximal Gene Expression in Hard-to-Transfect CellsExcellent Cell Viability & MorphologyHighly Efficient: Requires Minimal Reagent & DNAReady-to-Use ProtocolCost Effective
Lonza Walkersville P1 Primary Cell 4D-Nucleofector X Kit S
The P1 Primary Cell 4D-Nucleofector X Kit S is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL P1 Primary Cell Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μl)
Qiagen, Inc. EFFECTENE TRANSFCT.REAGENT 1ML
Effectene Transfection Reagent, no complex removal needed for most cell lines, fast and easy transfection, high efficiencies with a wide variety of cell types, contains: 1mL effective Reagent, Enhancer, Buffer; for 40 transfections in 60mm
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Lonza Walkersville Mouse Embryonic Fibroblast Nucleofector Kit 2
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Mouse Embryonic Fibroblast Nucleofector Kit 2 is one of our two kits suited for efficient transfectiion of primary mouse embyonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells -Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Embryonic Fibroblasts0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Zymo Research Corporation Rattler™ Plating Beads (230 g) (5 Bottles) ^
The sterile glass beads are simply poured onto solid plated medium together with a liquid cell suspension, and the mixture is shaken to distribute the cells evenly over the medium's surface. This allows for numerous plates to be processed quickly and efficiently. Pour the Rattler Beads onto a series of plates, stack, and shake simultaneously in a side-to-side motion. The beads can be easily removed following inversion of the plates and pouring off from the plate lids. Using the Rattler Plating Beads is simple, easy, and saves you time. The beads come sterile in polycarbonate bottles and can be reused following cleaning and washing.Rattler™ Plating Beads Fast & Easy-To-Use: Simply pour beads onto the plate, and shake to quickly spread cells evenly over the entire surface. Ideal for processing multiple plates simultaneously. No Flaming Required: 4.5 mm glass plating beads are provided sterile in polycarbonate bottles. Reusable: Just clean and autoclave after use.
OriGene TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial)
TurboFectin Transfection Reagent (1 mL in 1 vial)Components: One (1) Vial TurboFectin Transfection Reagent (1.05 mL) 1 TurboFectin Features: Wide cell spectrum: valided on >100 cell lines and primary cells, view list, Very low cell toxicity, Simplified use: compatible with serum and antibiotics in the mediaQuality Control: Each batch of TurboFectin Transfection Reagent has been tested and yields at least 8 ng of luciferase per 35 mm well after transfection into NIH3T3 cells. TurboFectin reagent is supplied in ethanol to ensure sterilityStability: The transfection reagent is stable for one (1) year from date of shipping when stored at +4°C.Storage: Upon receipt, please store the TurboFectin Transfection Reagent at +4°C.Type: Transfection Reagent
Lonza Walkersville Human Stem Cell Nucleofector Kit 1
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 1 is one of our two kits suited for efficient transfection of human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 1, 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit L
The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL P3 Primary Cell Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessels12 x Single use pipettes
New England Biolabs, Inc. NEB® 10-beta Competent E. coli (High Efficiency) – 6 x 0.2 ml/tube
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Lonza Walkersville SG Cell Line 4D-Nucleofector X Kit S
The SG Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells Medium throughput applicationsOptimization of transfection conditions, e.g. substrate titrationsContent:1 x 0.675 mL SG Cell Line Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μl)